evt basal media Search Results


evt basal media  (Thermo Fisher)
99
Thermo Fisher evt basal media
Evt Basal Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/evt basal media/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
evt basal media - by Bioz Stars, 2026-04
99/100 stars
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evt basal medium  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc evt basal medium
Figure 3. Directed differentiation of EVTs and STBs from naı¨ve <t>hPSC-derived</t> <t>hTSCs.</t> (A) The experimental scheme for <t>EVT</t> differentiation from naı¨ve hPSC-derived hTSCs. (B) Phase contrast image of H9 and AN_1.1 hTSC-like and EVT-like cells. The scale bars indicate 75 mm. (C) Flow cytometry analysis for EVT marker HLA-G in H9 and AN_1.1 hTSC-like cells and EVT-like cells. (D) Levels of MMP2 secreted by H9 and AN_1.1 hTSC-like cells and EVT-like cells as measured by ELISA. Error bars indicate ±1 SD of technical replicates. (E) Quantitative gene expression analysis for EVT marker gene Figure 3 continued on next page
Evt Basal Medium, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/evt basal medium/product/Cell Signaling Technology Inc
Average 93 stars, based on 1 article reviews
evt basal medium - by Bioz Stars, 2026-04
93/100 stars
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dmem/f12  (Thermo Fisher)
90
Thermo Fisher dmem/f12
Figure 3. Directed differentiation of EVTs and STBs from naı¨ve <t>hPSC-derived</t> <t>hTSCs.</t> (A) The experimental scheme for <t>EVT</t> differentiation from naı¨ve hPSC-derived hTSCs. (B) Phase contrast image of H9 and AN_1.1 hTSC-like and EVT-like cells. The scale bars indicate 75 mm. (C) Flow cytometry analysis for EVT marker HLA-G in H9 and AN_1.1 hTSC-like cells and EVT-like cells. (D) Levels of MMP2 secreted by H9 and AN_1.1 hTSC-like cells and EVT-like cells as measured by ELISA. Error bars indicate ±1 SD of technical replicates. (E) Quantitative gene expression analysis for EVT marker gene Figure 3 continued on next page
Dmem/F12, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dmem/f12/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
dmem/f12 - by Bioz Stars, 2026-04
90/100 stars
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2 ml evt basal medium  (Thermo Fisher)
90
Thermo Fisher 2 ml evt basal medium
( A ) The experimental scheme for <t>EVT</t> differentiation from naïve <t>hPSC-derived</t> <t>hTSCs.</t> ( B ) Phase contrast image of H9 and AN_1.1 hTSC-like and EVT-like cells. The scale bars indicate 75 μm. ( C ) Flow cytometry analysis for EVT marker HLA-G in H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( D ) Levels of MMP2 secreted by H9 and AN_1.1 hTSC-like cells and EVT-like cells as measured by ELISA. Error bars indicate ±1 SD of technical replicates. ( E ) Quantitative gene expression analysis for EVT marker gene MMP2 in H9 and AN_1.1 hTSC-like cells and EVT-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001. ( F ) Matrigel invasion assay of H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( G ) The experimental scheme for STB differentiation from naïve hPSC-derived hTSCs. ( H ) Phase contrast image of H9 and AN_1.1 hTSC-like and 3D STB-like cells. The scale bars indicate 75 μm. ( I ) Quantitative gene expression analysis for STB marker genes CGB and SDC1 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ±1 SD of technical replicates. ‘*' indicates a p-value<0.05, and ‘**' indicates a p-value<0.01. ( J ) Immunofluorescence staining for STB markers hCG and SDC1 in H9 3D STB-like cells. The scale bars indicate 75 μm. ( K ) Immunofluorescence staining for STB markers hCG and SDC1 in AN_1.1 3D STB-like cells. The scale bars indicate 75 μm. ( L ) Quantitative gene expression analysis for TSC marker gene TEAD4 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001.
2 Ml Evt Basal Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2 ml evt basal medium/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
2 ml evt basal medium - by Bioz Stars, 2026-04
90/100 stars
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evt basal medium  (Corning Life Sciences)
90
Corning Life Sciences evt basal medium
( A ) The experimental scheme for <t>EVT</t> differentiation from naïve <t>hPSC-derived</t> <t>hTSCs.</t> ( B ) Phase contrast image of H9 and AN_1.1 hTSC-like and EVT-like cells. The scale bars indicate 75 μm. ( C ) Flow cytometry analysis for EVT marker HLA-G in H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( D ) Levels of MMP2 secreted by H9 and AN_1.1 hTSC-like cells and EVT-like cells as measured by ELISA. Error bars indicate ±1 SD of technical replicates. ( E ) Quantitative gene expression analysis for EVT marker gene MMP2 in H9 and AN_1.1 hTSC-like cells and EVT-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001. ( F ) Matrigel invasion assay of H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( G ) The experimental scheme for STB differentiation from naïve hPSC-derived hTSCs. ( H ) Phase contrast image of H9 and AN_1.1 hTSC-like and 3D STB-like cells. The scale bars indicate 75 μm. ( I ) Quantitative gene expression analysis for STB marker genes CGB and SDC1 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ±1 SD of technical replicates. ‘*' indicates a p-value<0.05, and ‘**' indicates a p-value<0.01. ( J ) Immunofluorescence staining for STB markers hCG and SDC1 in H9 3D STB-like cells. The scale bars indicate 75 μm. ( K ) Immunofluorescence staining for STB markers hCG and SDC1 in AN_1.1 3D STB-like cells. The scale bars indicate 75 μm. ( L ) Quantitative gene expression analysis for TSC marker gene TEAD4 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001.
Evt Basal Medium, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/evt basal medium/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
evt basal medium - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Figure 3. Directed differentiation of EVTs and STBs from naı¨ve hPSC-derived hTSCs. (A) The experimental scheme for EVT differentiation from naı¨ve hPSC-derived hTSCs. (B) Phase contrast image of H9 and AN_1.1 hTSC-like and EVT-like cells. The scale bars indicate 75 mm. (C) Flow cytometry analysis for EVT marker HLA-G in H9 and AN_1.1 hTSC-like cells and EVT-like cells. (D) Levels of MMP2 secreted by H9 and AN_1.1 hTSC-like cells and EVT-like cells as measured by ELISA. Error bars indicate ±1 SD of technical replicates. (E) Quantitative gene expression analysis for EVT marker gene Figure 3 continued on next page

Journal: eLife

Article Title: Derivation of trophoblast stem cells from naïve human pluripotent stem cells

doi: 10.7554/elife.52504

Figure Lengend Snippet: Figure 3. Directed differentiation of EVTs and STBs from naı¨ve hPSC-derived hTSCs. (A) The experimental scheme for EVT differentiation from naı¨ve hPSC-derived hTSCs. (B) Phase contrast image of H9 and AN_1.1 hTSC-like and EVT-like cells. The scale bars indicate 75 mm. (C) Flow cytometry analysis for EVT marker HLA-G in H9 and AN_1.1 hTSC-like cells and EVT-like cells. (D) Levels of MMP2 secreted by H9 and AN_1.1 hTSC-like cells and EVT-like cells as measured by ELISA. Error bars indicate ±1 SD of technical replicates. (E) Quantitative gene expression analysis for EVT marker gene Figure 3 continued on next page

Article Snippet: 0.75 105 hTSCs were seeded per well in 2 mL EVT basal medium [DMEM/F12 supplemented with 0.1 mM b-mercaptoethanol, 0.5% penicillin-streptomycin, 0.3% BSA, 1% ITS-X, 7.5 mM A83-01, 2.5 mM Y27632] supplemented with 4% KSR (Gibco, 10828) and 100 ng/mL NRG1 (Cell signaling, 5218SC).

Techniques: Derivative Assay, Flow Cytometry, Marker, Enzyme-linked Immunosorbent Assay, Gene Expression

( A ) The experimental scheme for EVT differentiation from naïve hPSC-derived hTSCs. ( B ) Phase contrast image of H9 and AN_1.1 hTSC-like and EVT-like cells. The scale bars indicate 75 μm. ( C ) Flow cytometry analysis for EVT marker HLA-G in H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( D ) Levels of MMP2 secreted by H9 and AN_1.1 hTSC-like cells and EVT-like cells as measured by ELISA. Error bars indicate ±1 SD of technical replicates. ( E ) Quantitative gene expression analysis for EVT marker gene MMP2 in H9 and AN_1.1 hTSC-like cells and EVT-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001. ( F ) Matrigel invasion assay of H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( G ) The experimental scheme for STB differentiation from naïve hPSC-derived hTSCs. ( H ) Phase contrast image of H9 and AN_1.1 hTSC-like and 3D STB-like cells. The scale bars indicate 75 μm. ( I ) Quantitative gene expression analysis for STB marker genes CGB and SDC1 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ±1 SD of technical replicates. ‘*' indicates a p-value<0.05, and ‘**' indicates a p-value<0.01. ( J ) Immunofluorescence staining for STB markers hCG and SDC1 in H9 3D STB-like cells. The scale bars indicate 75 μm. ( K ) Immunofluorescence staining for STB markers hCG and SDC1 in AN_1.1 3D STB-like cells. The scale bars indicate 75 μm. ( L ) Quantitative gene expression analysis for TSC marker gene TEAD4 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001.

Journal: eLife

Article Title: Derivation of trophoblast stem cells from naïve human pluripotent stem cells

doi: 10.7554/eLife.52504

Figure Lengend Snippet: ( A ) The experimental scheme for EVT differentiation from naïve hPSC-derived hTSCs. ( B ) Phase contrast image of H9 and AN_1.1 hTSC-like and EVT-like cells. The scale bars indicate 75 μm. ( C ) Flow cytometry analysis for EVT marker HLA-G in H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( D ) Levels of MMP2 secreted by H9 and AN_1.1 hTSC-like cells and EVT-like cells as measured by ELISA. Error bars indicate ±1 SD of technical replicates. ( E ) Quantitative gene expression analysis for EVT marker gene MMP2 in H9 and AN_1.1 hTSC-like cells and EVT-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001. ( F ) Matrigel invasion assay of H9 and AN_1.1 hTSC-like cells and EVT-like cells. ( G ) The experimental scheme for STB differentiation from naïve hPSC-derived hTSCs. ( H ) Phase contrast image of H9 and AN_1.1 hTSC-like and 3D STB-like cells. The scale bars indicate 75 μm. ( I ) Quantitative gene expression analysis for STB marker genes CGB and SDC1 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ±1 SD of technical replicates. ‘*' indicates a p-value<0.05, and ‘**' indicates a p-value<0.01. ( J ) Immunofluorescence staining for STB markers hCG and SDC1 in H9 3D STB-like cells. The scale bars indicate 75 μm. ( K ) Immunofluorescence staining for STB markers hCG and SDC1 in AN_1.1 3D STB-like cells. The scale bars indicate 75 μm. ( L ) Quantitative gene expression analysis for TSC marker gene TEAD4 in H9 and AN_1.1 hTSC-like cells and 3D STB-like cells. Error bars indicate ± 1 SD of technical replicates. ‘***' indicates a p-value<0.001.

Article Snippet: 0.75 × 10 5 hTSCs were seeded per well in 2 mL EVT basal medium [DMEM/F12 supplemented with 0.1 mM β-mercaptoethanol, 0.5% penicillin-streptomycin, 0.3% BSA, 1% ITS-X, 7.5 μM A83-01, 2.5 μM Y27632] supplemented with 4% KSR (Gibco, 10828) and 100 ng/mL NRG1 (Cell signaling, 5218SC).

Techniques: Derivative Assay, Flow Cytometry, Marker, Enzyme-linked Immunosorbent Assay, Expressing, Invasion Assay, Immunofluorescence, Staining